Correlation of Density and Water Quality to Cortisol of Cantang Grouper Fish (Epinephelus fuscoguttatus X Epinephelus lanceolatus) in Floating Net Cages

Cantang grouper is a hybrid resulting from crosses between female tiger (E. fuscoguttatus) and male kertang lanceolatus). Cultivation with floating net cages one of the attempts made to cultivate cantang fish. Problems that arise are non-uniformity size fish when harvesting, this problem can lead decrease in selling prices maintenance tends be longer. Metabolic disorders living beings caused due physiological stress causes water quality. Water quality closely related feeding stocking density because increase feed affect accumulation feces which slow growth. This study was conducted find out influence factors play most important role based on indicators cortisol levels. Cortisol testing performed using elisa method Bioassay Technology Laboratory E0014Fi kit type. Data processed multiple linear regression test analysis serves determine relationship free variable (independent) X non-free variables (dependent) Y. Regression used causal relationships. After test, then Analysis Variance (ANNOVA) conducted, difference dependent parameters significantly independent. There strong correlation simultaneously levels nets, as seen R value 0.546. The influential factor nitrite an effect 19.7%. next significant ammonia 37.1%. indicated known not state increased NO2 NH3. Density has no its significance above (p>0.05).